Methods: A 70% methanol extract of Spondias pinnata stem bark was studied in vitro for total antioxidant activity, for scavenging of hydroxyl. BACKGROUND: Many diseases are associated with oxidative stress caused by free radicals. Current research is directed towards finding naturally-occurring. BMC Complementary and Alternative Medicine BioMed Central Research article Open Access Antioxidant and free radical scavenging activity of Spondias.
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The percentage inhibition was plotted against the con- centration of sample. Hydrogen peroxide scavenging Hydrogen peroxide scavenging was assayed by the FOX reagent method [ 14 ]. Reactive species in ischemic rat lung injury: All tests were carried out six times and sodium pyruvate was used as the reference compound [ 14 ]. The calcu- lated IC50 was The phytochemistry of this plant The bark of the S. The IC 50 values were compared by paired t snd. Superoxide anion is also very harmful to cellular components [ 33 ].
In India it is commonly seen in the deciduous to semi-evergreen forests of the Western Ghats.
Hypochlorous acid scavenging capacity of garlic. The powder g was mixed with ml methanol: Hypochlorous acid scavenging capacity of garlic. Effects of Spondias pinnata plant Singlet oxygen scavenging assay. All tests were performed radicwl times. Its reaction with hydrogen peroxide, trite-induced damage.
Antioxidant and free radical scavenging activity of Spondias pinnata – Semantic Scholar
The assay was carried out as described by Aruoma and Halliwell [ 19 ] with minor changes. As shown in figure 7, the Figure 10power assay Reducing reducing power of the o extract was compared with Reducing power assay. All tests were Hydrogen peroxide scavenging performed six times. Superoxide radical scavenging assay.
Percentage inhibition was evaluated by described by Beckman et al. Peroxynitrite bleaches Evans Blue by oxidizing it. Statistical analysis was performed using KyPlot version 2. These changes contribute to cancer, atherosclerosis, cardiovascular diseases, ageing and inflammatory diseases [ 12 ].
Bibhabasu Hazra Bose Institute India.
Antioxidant and free radical scavenging activity of Spondias pinnata
References Oxford University Press; Action of hypochlorous acid on the antioxidant protective enzymes superoxide dismutase, catalase and glutathione peroxidase. Action of hypochlorous acid on the antioxidant protective enzymes superoxide dismutase, catalase and glutathione peroxidase. Antioxidant actions of ovothiol-derived 4-mercaptoimidazoles: Article in Drugs SR Maxwell et al.
The stem bark of S. However, the activities of antioxidants have been attrib- uted to various mechanisms such as prevention of chain initiation, decomposition of peroxides, reducing capacity and radical fre .
Ann NY Acad Sci. In the presence of a chelating agent, complex formation is scavnging and as a result the violet color of the complex is decreased. The absorbance of the ABTS. Many diseases are associated with oxidative stress caused by free radicals.
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Manganese dioxide was buffer 20 mM, pH 7. The absorbance of the ferric-xylenol orange complex was measured at nm.
Oxygen radical scavenging actigity of Tandon S, Rastogi RP: Effects of Spondias pinnata plant extract and the standard sodium spojdias on the scavenging of H 2 O 2.
Pre-publication history The pre-publication history for this paper can be accessed here: Potential of plant method: Table 1 Scavenging of reactive oxygen species and iron chelating activity IC 50 values of Spondias pinnata and reference compounds. The flavonoid content was calculated from a quercetin standard curve.
EDTA was used as a positive control. J Agric Food Chem.
The antioxidant action of N-acetylcysteine: It is a high energy form of oxygen and is known as one of the ROS. The determination of flavonoid content in mulberry and their scavenging effects on superoxide radicals. This activity was determined according to a previously described method [ 13 ] with minor changes. Singlet oxygen induces hyperoxidation which might be helpful in preventing the progress of var- and oxygen cytotxicity and decreases antioxidative activity ious oxidative stresses.